Sickle Hemoglobin: A Specific Radioimmunoassay

Sickle hemoglobin: a specific radioimmunoassay.

For the quantitation of hemoglobin S, a radioimm unoassay has been developed which is specific and highly sensitive. Hemoglobin S was purified by column chromatography and injected with complete Freund’s adjuvant into goats. Each goat serum was tested for reactivity against hemoglobins A and S by immunodiffusion and by quantitative precipitation. Hemoglobin A reactivity was removed by absorptio...

Hemoglobin sickle D Punjab — a case report

Compound heterozygosity for βS/βD results in a severe hemolytic anemia and a clinical syndrome similar to that of sickle cell disease. Here, we report a case of HbSD Punjab disease. A 10 year old female child residing at Nagpur, Maharashtra presented with severe hemolytic anemia, hepatosplenomegaly and occasional pains in bones and abdomen. Initially, she was thought to be a case of sickle cell...

Denatured hemoglobin in sickle erythrocytes.

To study the nature of numerous inclusion bodies seen in red cells from patients with sickle cell disease (Hb SS), we have prepared red cell ghosts free of oxyhemoglobin and analyzed them by spectrophotometric and heme extraction methods. The absorption spectrum in the visible region of the ghost suspensions was typical of hemichromes. The spectrum was similar to that of denatured hemoglobin re...

A SPECIFIC, SENSITIVE, AND ONE-STEP EXTRACTION RADIOIMMUNOASSAY OF TESTOSTERONE FOR CLINICAL DIAGNOSIS

Testosterone was measured using antibodies raised against testosterone II B-carboxymethyl ether bovine serum albmin (T-IIB-CME-BSA) and testosterone 3- O-carboxymethyl oxime-BSA as immunogen. The antibody produced in this study exhibits minimal cross reactivity with the structurally related steroids specially 5 dihydrotestosterone (5 DHD. This allows to ommit the clean up step and measure ...

Intraerythrocytic Hemoglobin Crystals in Sickle Cell-hemoglobin C Disease.